Selection of stable housekeeping genes for gene expression studies in different varieties of black pepper (Piper nigrum L.)

Real Time quantitative PCR (RT-qPCR) is a widely used technique to study the transcript level modulation of genes during developmental stages crop plants as well in stress responses. Suitable reference have not been validated many including black pepper. In this study, expression stability six commonly housekeeping viz., actin, ?-tubulin, elongation factor, initiation ubiquitin and glyceraldehyde 3- phosphate dehydrogenase were evaluated by RT-qPCR growth pepper inflorescence varieties Panniyur 1, Karimunda Thekken. The results analyzed using geNorm Normfinder statistical algorithms. Stable gene critical for accurate normalization target data RT-qPCR. factor identified most stable different Thekken, 1 respectively. Actin combination with GAPDH obtained optimal It first report on identification can aid studies yield improvement. will